Hami melon ‘Queen’ (Cucumis melo ssp. melo var. ameri Pangalo) is the most widely cultivated and exported type of melon in Xinjiang Province, Northwest China. We previously found the unique traits of Hami melon ‘Queen’ for wave seeds and tight-placenta fruits. An analysis of the inheritance showed that these traits were controlled by two recessive genes wave seed (ws) gene and tight-placenta (tp) gene, respectively. Here, to identify these two traits and melon seed–related traits, segregation populations including BC_{step step one} and F_dos derived from a cross between ‘Queen’ (P₁) and MR-1 (P₂) were used as mapping populations. Eighty-seven simple sequence repeat (SSR) markers were used in map construction of BC₁P₁ population, and as a result, ws and tp were identified on linkage group 1. Analysis of quantitative trait locus (QTL) referring seed traits showed that QTL ss1.1 for seed shape (SS) and QTL sl1.1 for seed length (SL) were located at LG 1, supported by likelihood of odds (LODs) of 15.6 and 13.4, respectively, and both linked with ws. Subsequently, the genetic linkage and parental re-sequence analysis were constructed for fine mapping ws and tp. Genetic analysis showed that ws and tp were located in CM3.5_scaffold00060 on LG 1, flanked by InDelchr1-3241 and InDelchr1-3233. The 80.9-kb physical distance of this region included 11 candidate genes. Among them, MELO3C023549 and MELO3C023551 could be candidates for ws and tp by sequence alignment and allelic variation survey in parental lines. MELO3C023549 was predicted to encode an MYB46-like transcription factor related to positive regulation of secondary cell wall biogenesis. MELO3C023551 was annotated to encode a cellulose synthase A (CESA) associated with cellulose biosynthetic process.

Abstract

Hami melon ‘Queen’ (Cucumis melo ssp. melo var . ameri Pangalo) is the most widely cultivated and exported type of melon in Xinjiang Province, Northwest China. We previously found the unique traits of Hami melon ‘Queen’ for wave seeds and tight-placenta fruits. An analysis of the inheritance showed that these traits were controlled by two recessive genes wave seed (ws) gene and tight-placenta (tp) gene, respectively. Here, to identify these two traits and melon seed–related traits, segregation populations including BC₁ and F₂ derived from a cross between ‘Queen’ (P₁) and MR-1 (P₂) were used as mapping populations. Eighty-seven simple sequence repeat (SSR) markers were used in map construction of BC₁P₁ population, and as a result, ws and tp were identified on linkage group 1. Analysis of quantitative trait locus (QTL) referring seed traits showed that QTL ss1.1 for seed shape (SS) and QTL sl1.1 for seed length (SL) were located at LG 1, supported by likelihood of odds (LODs) of 15.6 and 13.4, respectively, and both linked with ws. Subsequently, the genetic linkage and parental re-sequence analysis were constructed for fine mapping ws and tp. Genetic analysis showed that ws and tp were located in CM3.5_scaffold00060 on LG 1, flanked by InDelchr1-3241 and InDelchr1-3233. The 80.9-kb physical distance of this region included 11 candidate genes. Among them, MELO3C023549 and MELO3C023551 could be candidates for ws and tp by sequence alignment and allelic variation survey in parental lines. MELO3C023549 was predicted to encode an MYB46-like transcription factor related to positive regulation of secondary cell wall biogenesis. MELO3C023551 was annotated to encode a cellulose synthase A (CESA) associated with cellulose biosynthetic process.

Inheritance and you can Unit Mapping regarding Rigorous-placenta Gene and Vegetables Characteristics when you look at the Chinese Hami Melon ‘Queen’

Melon (C. melo L.; 2n = 2x = 24), a primary financial good fresh fruit crop, is actually grown commonly inside the exotic and you will subtropical regions. Hami melon ‘Queen’ (C. melo ssp. melo var. ameri Pangalo) is considered the most commonly cultivated and you will shipped types of melon inside Xinjiang State, Northwest China. The creation of nice Hami melon benefits from the new climate there, and additionally dead climate, large fluctuation of daily temperatures, good sunrays, and you may a lot of time sunlight cycle during the summer. Xinjiang Hami melon has higher reputation around the globe because of its book flavor and you will high sugar content (Ning ainsi que al., 2014b).